Analysis of staphylococcal enterotoxin A in milk by matrix-assisted laser desorption/ionization-time of flight mass spectrometry

Staphylococcal enterotoxin A (SEA) is an exotoxin excreted mainly by Staphylococcus aureus and nowadays is the most prevalent compound in staphylococcal food poisoning worldwide. SEA is highly heat-resistant, and usual cooking times and temperatures are unlikely to completely inactivate it. A procedure for extraction of this toxin based on protein precipitation with a mixture of dichloromethane and acidified water was used before SDS-PAGE separation of soluble proteins. Finally, bands of interest were excised from the gel and in-gel enzymatic digestion was done. SEA from pasteurized milk was detected with matrix-assisted laser-desorption/ionization-time of flight (MALDI-TOF) mass spectrometry. Nineteen peptides (range 800-2400 Da) were identified as products of trypsin cleavage of the SEA standard with a score of 204 and 73% coverage of the protein sequence, whereas thirteen peptides were revealed for SEA extracted from milk with a score of 148 and 58% sequence coverage obtained. This procedure has been applied successfully for identification of SEA in milk.     

Weakly coercive mappings sharing a value

Some sufficient conditions are provided that guarantee that the difference of a compact mapping and a proper mapping defined between any two Banach spaces over $ \mathbb{K} $ \mathbb{K} has at least one zero. When conditions are strengthened, this difference has at most a finite number of zeros throughout the entire space. The proof of the result is constructive and is based upon a continuation method.     

Recent optimization models and trends in location,relocation, and dispatching of emergency medical vehicles

Over the past 10 years, a considerable amount of research has been devoted to the development of models to support decision making in the particular yet important context of Emergency Medical Services (EMS). More specifically, the need for advanced strategies to take into account the uncertainty and dynamism inherent to EMS, as well as the pertinence of socially oriented objectives, such as equity, and patient medical outcomes, have brought new and exciting challenges to the field. In this context, this paper summarizes and discusses modern modeling approaches to address problems related to ambulance fleet management, particularly those related to vehicle location and relocation, as well as dispatching decisions. Although it reviews early works on static ambulance location problems, this review concentrates on recent approaches to address tactical and operational decisions, and the interaction between these two types of decisions. Finally, it concludes on the current state of the art and identifies promising research avenues in the field.     

Quantum mechanics: cleaning up metaphysical baggage

The dynamic electronic structure of atoms and molecules can be directly observed by means of the (e, 2e) reaction, which measures the distribution of energies and momenta of two electrons in coincidence after a knockout reaction initiated by an electron beam of known momentum incident on a molecular gas target. The molecular state for each event is identified by the electron separation energy. The recoil momentum for each event is known from the difference of measured initial and final momenta. It has been verified that values of this momentum are equal under suitable conditions to the momentum of the electron in the target immediately before knockout. Thus the spherically-averaged electron momentum distribution for each molecular orbital is measured. This is directly related to molecular orbitals calculated by the methods of quantum chemistry. Properties obtained by this method for different types of molecules are discussed.     

X‐ray absorption study of the local structure at the NiO/oxide interfaces

This work reports an X‐ray absorption near‐edge structure (XANES) spectroscopy study at the Ni K‐edge in the early stages of growth of NiO on non‐ordered SiO₂, Al₂O₃ and MgO thin films substrates. Two different coverages of NiO on the substrates have been studied. The analysis of the XANES region shows that for high coverages (80 Eq‐ML) the spectra are similar to that of bulk NiO, being identical for all substrates. In contrast, for low coverages (1 Eq‐ML) the spectra differ from that of large coverages indicating that the local order around Ni is limited to the first two coordination shells. In addition, the results also suggest the formation of cross‐linking bonds Ni—O—M (M = Si, Al, Mg) at the interface.     

Diversification strategies in tabu search algorithms for the maximum clique problem

The purpose of this study is to develop some understanding of the benefits that can be derived from the inclusion of diversification strategies in tabu search methods. To do so, we discuss the implementation of various diversification strategies in several tabu search heuristics developed for the maximum clique problem. Computational results on a large set of randomly generated test problems are reported and compared to assess the impact of these techniques on solution quality and running time.     

A tabu search procedure for multicommodity location/allocation with balancing requirements

We propose a tabu search heuristic for the location/allocation problem with balancing requirements. This problem typically arises in the context of the medium term management of a fleet of containers of multiple types, where container depots have to be selected, the assignment of customers to depots has to be established for each type of container, and the interdepot container traffic has to be planned to account for differences in supplies and demands in various zones of the geographical territory served by a container shipping company. It is modeled as a mixed integer program, which combines zero-one location variables and a multicommodity network flow structure. Extensive computational results on a set of benchmark problems and comparisons with an efficient dual ascent procedure are reported. These show that tabu search is a competitive approach for this class of problems.     

Classes Logarithmiques Au Sens Restreint

In this paper we introduce the concept of logarithmic 2-classes in the narrow sense for a number field. Then we obtain fix point formulae for a cyclic 2-extension of this kind of fields.      

New antitumour active platinum compounds containing carboxylate ligands in trans geometry: synthesis, crystal structure and biological activity

New asymmetric trans-platinum(II) complexes, composed of an isopropylamine, an azole and two carboxylate leaving groups, are presented. The crystal and molecular structures of one of the complexes has been determined and the cytotoxicity and reactivity with 5'-guanosine monophosphate is reported. The complexes show a reduced reactivity, but no decrease in cytotoxic activity compared to their chloro-counterparts. Furthermore the complexes largely overcome cisplatin resistance, they therefore present an interesting class of antitumour active trans-platinum complexes.     

Shared value in a finite-dimensional Banach manifold

Sufficient conditions are given to assert that two C¹-mappings share only one value in a connected compact Banach manifold modelled over ?ⁿ. The proof of the result, which is based upon continuation methods, is constructive.